The P2Y purinoceptor in rat brain microvascular endothelial cells couple to inhibition of adenylate cyclase
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چکیده
منابع مشابه
Detergent-dispersed Adenylate Cyclase from Rat Brain
Some properties of particulate and detergent-dispersed preparations of adenylate cyclase from rat brain have been studied. The activity of the particulate enzyme was greater with Mn2f than with Mg2+ and the activity in the presence of either cation was enhanced by For nonionic detergents. In the presence of the non-ionic detergent Lubrol-PX, enzyme activity was further enhanced by dithiothreito...
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Clopidogrel, like the homologous thienopyridine derivative tidopidine, selectively inhibits platelet aggregation induced by ADP. We have previously described two nucleotide-binding sites on platelets related to ADPmediated platelet responses. The first is a high-affinity binding she for 2-methylthio-ADP (2-MeSADP) that is linked to the inhibition of stimulated adenylate cyclase. The second is t...
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Gossypol, a polyphenolic binaphthalene -dialdehyde reputed to exert contraceptive action in males, reversibly inhibits adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] in a concentration-dependent manner. In membranes prepared from a variety of organs, the half-maximal inhibitory concentration (IC50) ranges from 75 microM (rat Leydig tumor cells) to 250 microM (rat liver memb...
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High-affinity muscarinic cholinergic receptors were detected in myelin purified from rat brain stem with use of the radioligands 3H-N-methylscopolamine (3H-NMS), 3H-quinuclidinyl benzilate (3H-QNB), and 3H-pirenzepine. 3H-NMS binding was also present in myelin isolated from corpus callosum. In contrast, several other receptor types, including alpha 1- and alpha 2-adrenergic receptors, present i...
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Human platelet membranes contain a2 adrenergic receptors which mediate the guanine nucleotide-dependent inhibition of adenylate cyclase induced by epinephrine. Previous investigations have provided insight into the mechanisms of adenylate cyclase stimulation by studying hormone-induced release of radiolabeled guanine nucleotides from membranes preincubated with the nucleotides. In the present s...
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ژورنال
عنوان ژورنال: British Journal of Pharmacology
سال: 1996
ISSN: 0007-1188
DOI: 10.1111/j.1476-5381.1996.tb16050.x